How Does Work?

Retention of any compound in HPLC is affected by the values of chromatographic conditions such as: Organic solvent %, Column temperature, Buffer pH, gradient time…etc.
Each of these conditions affects retention time of any compound through published model equations (either empirical or mechanistic).
uses the data resulting from few runs performed at different values of one or two method conditions to solve these equations for each compound.
After that apply these solved equations to all proposed values of the studied condition(s) to predict retention times of each compound as well as resolution between peaks.
The predicted resolution values are plotted as resolution maps (either 1D or 2D).
This map is interactive and produce the chromatogram corresponding to the point you click on.

CHROMOCAD

features

1. Do less, obtain more: Starting with two trial runs, you can obtain complete prediction map
2. All models included: Supports a wide range of chromatographic parameters and their combinations
3. Your lab: anywhere; experience the cloud-based operation
4. Training, technical support and method development services
6. A subscription plan suitable to your budget
6. Start your demo trial
7. Subscribe now

With only very few experimental runs, you can obtain a full and precise prediction of retention and resolution of your separation method

Do less, obtain more: Starting with two trial runs, you can obtain complete prediction map

Two or three trial runs were only required with working with CHROMOCAD with high accracy in predicted results. Other approaches may cause you much mor trials with prediction accuracy less than that obtained with CHROMOCAD. 

In isocratic runs, Only two experimental runs enable prediction of full chromatograms at any isocratic separation at any given %B (oganic solvent %) in mobile phase.

This feature is of great importance as this is the fastest way to search optimum conditions in chromatography. Furthermore, as the first principle mechanestic equations are used, this method produces the most accurate predictions. 

The most effective models are available in gradient elution HPLC:
Only two experimental gradient runs enable prediction of any gradient run as a function of variation of any of the following parameters:
  • Gradient time
  • Initial organic solvent % in gradient.
  • Final organic solvent % in gradient.
  • Flow rate
Only the mechanistic equations applied in CHROMOCAD are able to perform this task.
Using ordinary experimental design software packages will require huge number of runs (more than 30 runs in case of gradient elution modeling) while producing less accuracy than that obtained from CHROMOCAD.
 
CHROMOCAD enables optimization of one factor (such as organic solvent %, pH, temperature,...). Optimization requires only 2-3 initial experimental runs differing in the value of the selected factor for optimization.

After performing the initial experiments, the retention data are entered into the software interface. This will result into a plot of minimum resolution between peaks as a function of the optmized method parameter. From this plot you can select the optimum condition. CHROMOCAD will predict the chromatogram at the selected condition.







CHROMOCAD enables simultaneous optimization of two different chromatographic parameters (e.g. % organic solvent and column temperature). CHROMOCAD will need only 4-9 initial runs.
CHROMOCAD help files will aid you to plan these trials. 

After performing the trials and entering their data in CHROMOCAD, you will obtain a 2D contour map with X and Y axes represent the optimized two parameters.

The color of each point in the map represents minimum resolution between peaks as indicated by color code legand. 

You can pick the optimum point by visual inspection of the map and CHROMOCAD will predict the chromatogram of this optimum conditions.
Simultaneous optimization of two different parameters



A large number of chromatographic parameters including gradient systems optimization and instrument variations are included as well as their combinations

All models included: Supports a wide range of chromatographic parameters and their combinations



CHROMOCAD supports the optimization of the following chromatographic conditions in 1D mode:
-- Organic solvent % (%B) in Reversed Phase Chromatography.
-- pH of buffer or mobile phase at constant ionic strength- both Henderson-Hazelbach or polynomial models.
-- Temperature.
-- Ion paring agent concentration or amine modifier at constant ionic strength.
-- Buffer concentration in cyano or amino columns.
-- Gradient time.
-- Ternary solvent ratio (ratio of the third organic solvent to the ratio of total organic solvent ratio.


Chromcad supports the following combinations of chromatographic parameters in 2D mode:
-- %B and pH
-- %B and Temperature
-- pH and Temperature
-- pH and Ion pairing agent concentration.
-- Gradient time and Temperature.
-- Gradient time and pH both Henderson-Hazelbach or polynomial models.
-- %B and Ternary solvent ratio.
-- Gradient time and Ternary solvent ratio.

Also CHROMOCAD support the influence of instrumental variations on gradient elution by applying changes of instrument dwell volume.




Cost effective and flexible cloud-based operation of CHROMOCAD

Your lab: anywhere; experience the cloud-based operation

Unlock the full potential of your chromatography lab with CHROMOCAD cloud-based solution. 
Our subscription based model offers a cost-effective alternative to buy-and-install software traditional approach. Our approach ensures that you get the most benefits of your investments.
Say goodbye to software incompatibility issues during installation and operation and embrass the seamless integration of our application. 
With CHROMOCAD you can access HPLC method development tools from any device , with any operating system,  anywhere at anytime.
Enjoy the benefits of always up to date software and freedom to work without the physical constraints of installation site. 
With CHROMOCAD you will experience reduced cost and increased productivity and more focus on achieving results.




We offer a comprehensive set of training programs and dedicated technical support to chromatgraphers. Special requests for methd development services are available also.

Training, technical support and method development services

Genernal training on HPLC method development
We provide different Training program on HPLC method development and other related topics to all experience levels.
We offer different styles of training: online streaming, On-demand , In-site and in our head-offices.
Check the following pruchore for the updated training programs (Training pruchore).
You can incquire and order any of our training programs from this form HPLC training inquiry form.
Once you submit this form you will be contacted for the selected course scheduled link to enrol according to your request. 

Training on CHROMOCAD software
We provide hands-on interactive training sessions on using of CHROMOCAD software. These session are arranged either online streaming, On-demand , In-site and in our head-offices.
in these hands-on; you can work with our trainers on the provided training account on software to get experience on computer aided method development.
Check the following Schedule for the updated Software training programs (CHROMOCAD training schedule).
You can book your seat for any of the upcoming courses.
Once you submit your response in this schedule you will be contacted for the selected course scheduled link to enrol according to your request.

Software help
The software package includes a comprehensive set of  help files to guide you step-by-step for the best use of the software to achieve you method development goals.

Technical support 
We offer technical support for use of software as well as the related method development issues. 
We provide adequate channels for technical support to suit our customer needs from different segements: individuals, teams or organizations.
You can access technical support by mail (Support@chromocad.com) as well as by the channels provided to you as a part of your supscription.

Custome method development services
Contact us through this form to inquire custome and cost effective HPLC computer aided method development service HPLC computer aided method development contract inquiry.
Once you submit the form you will be contacted to organize the method development tasks.
You will get the following output:
  • Method experimental conditions
  • Detailed protocol and report of development experiments
  • CHROMOCAD reports for development 
  • Original chromatograms and audit-trail of the development runs.



Different subscription options for individuals and organisations

A subscription plan suitable to your budget

    CHROMOCAD is provided as a web-based service through subscription. 

We provide different subscription plans with different time durations and features to suite each type of our customers:
  • Individual researchers
  • Multinational research groups 
  • Research institutions and universities 
  • Contract Research Organisations (CROs)
  • Chemical and pharmaceutical industry

     We can help you to select the best subscription option for your needs according to the nature of your project(s), customer segments and expected time to finish your task. We are keen to ensure that the selected plan will give you the highest benefits, with no extra cost.
     Just select the choices that fits your needs in the following form: Subscription Plan inquiry form
Once you submit the form,  our support will contact you within 24hr to customize with you the best subscription plan for your needs. 





Start your demo trial of CHROMOCAD

Start your demo trial

We offer a free 10 days trial for our CHROMOCAD software to evaluate the software suitability for your needs.
you can access your trial using this form (Start your demo trial).
We wish you good experience with our software



Subscribe to CHROMOCAD service

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Start your subscription to CHROMOCAD software services.
You can access and edit your selected subscription from this link Access and edit subscription
or contact us to customize your subscription through this form Inquire about subscription price quote
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